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KMID : 0545120070170121949
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 12 p.1949 ~ p.1954
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Variations in Protein Glycosylation in Hansenula polymorpha Depending on Cell Culture Stage
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Kim So-Young
Sohn Jung-Hoon
Pyun Yu-Ryang
Choi Eui-Sung
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Abstract
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A simple way to prevent protein hyperglycosylation in Hansenula polymorpha was found. When glucose oxidase from Aspergillus niger and carboxymethyl cellulase from Bacillus subtilis were expressed under the control of an inducible methanol oxidase (MOX) promoter using methanol as a carbon source, hyperglycosylated forms occurred. In contrast, MOX-repressing carbon sources (e.g., glucose, sorbitol, and glycerol) greatly reduced the extent of hyperglycosylation. Carbon source starvation of the cells also reduced the level of glycosylation, which was reversed to hyperglycosylation by the resumption of cell growth. It was concluded that the proteins expressed under actively growing conditions are produced as hyperglycosylated forms, whereas those under slow or nongrowing conditions are as short-glycosylated forms. The prevention of hyperglycosylation in the Hansenula polymorpha expression system constitutes an additional advantage over the traditional Saccharomyces cerevisiae system in recombinant production of glycosylated proteins
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KEYWORD
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Glycosylation, Hansenula polymorpha, glucose oxidase, CMCase
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